Poster Presentation
The 20th Fungal Genetics Conference at Asilomar
March 23-28, 1999
David E. A. Catcheside [1], J. Paul Rasmussen [1], P. Jane
Yeadon [1], Frederick J. Bowring [1], Elie Kato [2] and W. Dorsey Stuart
[2]
[1] School of Biological Sciences, Flinders University, Adelaide,
South Australia
[2] Neugenesis Corporation, San Carlos, California
Poster Presentation
The 20th Fungal Genetics Conference at Asilomar
March 23-28, 1999
Abstract: Recombination hotspots active in
meiosis provide a means of diversifying pairs of DNA sequences differing
at multiple sites. We have constructed plasmids permitting targeted
transfection of heterologous genes located between his-3 and
the cog hotspot in Neurospora, this
positioning enables enrichment of post-meiotic recombinants by selection
of progeny that have experienced recombination at his-3.
Figure 1. Vector Construction
Figure 2. Transfection
Figure 3. Selection for his+
Figure 4. Resolution
of heterokaryons
Figure 5. Diversified genes